Max Planck Gesellschaft

Facilities of the Molecular Biogeochemistry Group

  • Accelarated Solvent Extraction (ASE-200)
DIONEX Corp., Sunnyvale, USA
The ASE-200 is an automated technique used to extract organic compounds from solid and semisolid samples with liquid, organic or aqueous solvents. The ASE 200 uses patented technologies that accelerate the extraction of analytes from samples. Common solvents are used at elevated temperatures and pressures to increase the speed and efficiency of the extraction process.
  • Buchi SpeedExtractor (E-916)
Flawil, Switzerland
The Buchi extractor is an updated automatic extraction technique which is use to extract organic compounds from solid and semisolid samples with liquid, organic or aqueous solvents. The Speed Extractor is 6 times faster than other Pressurized Solvent Extractor (PSEs). This feature increase productivity by processing up to 6 samples in parallel. Common solvents are used at elevated temperatures and pressures to increase the speed and efficiency of the extraction process.
  • Gas Chromatography-Flame Ionization Detector (GC-FID)
Agilent Technologies, Palo Alto USA
Gas chromatography is a technique for separating chemical substances that relies on differences in partitioning behavior between a flowing mobile phase and a stationary phase to separate the components in a mixture. A Flame ionization detector (FID) consists of a hydrogen (H2)/air flame and a collector plate. The effluent from the GC column passes through the flame, which breaks down organic molecules and produces ions. The ions are collected on a biased electrode and produce an electrical signal. In our work group we use this equipment to separate and quantify mixtures of organic compounds like: PLFA, n-alkanes, PAH, among others.
  • Gas Chromatography- Flame Ionization Detector (GC-FID)
Agilent Technologies, 7890B GC-System
Separation and quantification of individual components can be performed using a gas chromatograph with flame ionization detection.
  • Gas Chromatography-Mass Spectrometer (GC-MS)
Agilent Technologies, 7890A GC-System; 220 Ion trap MS
This GC-MS system is used for Identification of volatile and semi-volatile individual organic compounds in complex mixtures. Within the mass spectrometer the molecules are ionized. Once the sample is fragmented it will then be detected. The Structural determination of unknown organic compounds is accomplished by matching their spectra with reference spectra.
  • Gas Chromatography -Isotopic Ratio Mass Spectrometer (GC-IRMS) for C analysis
HP5890 GC, Agilent Technologies, Palo Alto USA;
IRMS: Deltaplus XL, Finnigan MAT, Bremen, Germany
This Delta+XL instrument is coupled with a GC-combustion system for compound specific isotope analysis (13C/12C and 2H/H) from GC eluates. For simultaneous compound identification, 10% of the GC effluent is split off to a GCQ organic mass spectrometer. This instrument is hosted by IsoLab.
  • Gas Chromatography -Isotopic Ratio Mass Spectrometer (GC-IRMS) for dD analysis
IRMS: Delta V Plus Isotope Ratio MS, Thermo Fisher Scientific, Bremen, Germany;
GC: 7890A with Monitor FID, Agilent Technologies, Palo Alto, USA;
The Delta V Plus is coupled with a GC-Isolink and Conflo IV system. The GC-Isolink is the further development of the GC-Combustion. The system holds two microreactors for compound specific isotope analysis for 13C/12C and 2H/1H from GC eluates. This instrument is also hosted by IsoLab.
  • Preparation Gas Chromatography (PrepGC)
GC: 6890 with Monitor FID, Agilent Technologies, Palo Alto, USA;
PFC: Preparative Fraction Collector, Gerstel, Mülheim an der Ruhr, Germany;
The PrepGC is designed for automated collection of compounds or compound groups after gas chromatographic separation. The PFC-unit is equipped with six sample traps and one waste trap. This system works with a liquid N2 trap cooling.
  • High Performance Liquid Chromatography (HPLC-PDA 100)
DIONEX Corp., Sunnydale, USA
High-performance liquid chromatography is a technique used to separate a mixture of compounds with the purpose of identifying, quantifying or purifying the individual components of the mixture. We use it mainly for the analysis of polysaccharides and organic acids.
  • High Performance Liquid Chromatography with fraction collector
HPLC: Agilent Technologies 1200 Series, Agilent Technologies, Palo Alto USA
Preparative high-performance liquid chromatography is used to quantify, identify and purify an individual component of a complex mixture. The purified compounds will be subsequently analyzed for compound specific stable and radiocarbon isotopic compositions.
  • High Performance Liquid Chromatography-High Resolution Mass Spectrometry
HPLC: UltiMate 3000, Dionex Softron GmbH, Germering, Germany
MS: Orbitrap Elite, Thermo Fisher Scientific, Dreieich, Germany;
High-performance liquid chromatography is used separate individual components of a complex mixture. The separated compounds are detected by mass spectrometry. The mass spectrometer is also used for direct injection of samples and their structure elucidation.
  • High Performance Liquid Chromatography-Isotopic Ratio Mass Spectrometer (HPLC-IRMS)
HPLC: ThermoFinnigan LC-IsoLink system, Thermo Electron, Bremen, Germany;
IRMS: Deltaplus XP, Thermo Electron, Bremen, Germany
This HPLC-IRMS system is used for compound specific isotope analysis (13C/12C) of liquid samples (soil water, sugar extracts, chloroform fumigation extracts/soil microbial biomass, nucleic acids: DNA, RNA). Recent improvements have been the SEC hyphenation for isotope analysis of size classes of soil DOC and soil microbial biomass.
  • Thermogravimetric Analysis (TGA/SDTA851)
Mettler Toledo
Thermogravimetric analysis is a method to detect changes in physical and/or chemical properties of materials. Therefore mass loss is measured as a function of increasing temperature/time. Information on physical and chemical phenomena, such as vaporization, sorption, desolvation, oxidation or reduction can be detected by this. We run our TGA with synthetic air and Argon and use it to analyze differences in the carbonaceous composition of soils.
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