IMPRS-gBGC workshop 'Analytical Techniques'

Category: Skill course
0.2 CP per course day

This skill course provides insights into different analytical techniques which are applied to resolve key questions in global biogeochemistry and related earth system sciences. Please do not participate in a course module if you are already familiar with the technique. The modules will give introductions to the methods. They do not address users.


1.  Overview

Date: September/October, 2016
- MPI-BGC, Hans-Knöll-Str. 10
- Institute for Inorganic and Analytical Chemistry (IAAC), seminar room #127, Lessingstr. 8
- Institute of Photonic Technology (IPHT), Albert-Einstein-Straße 9
- Institute of Geosciences (IGW), Burgweg 11
- Institute of Microbiology, Neugasse 25
- Trace Gas Analysis and Gas Standard Preparation Flask and Calibration Laboratory (CAL-FCL), Kahlaische Str. 4


2.  Agenda

L = lecture, S = seminar, D = demonstration, P = Practical

Day & TimeTypeContentWho & Where
Tue, Sept 13 Analytical Techniques in MicrobiologyKatrin Krause (Microbiology, FSU Jena)
9.00-10.00 amLIntroduction fungi and fluorescence microscopySeminar room (Neugasse 25)
10.00-12.00 amP(Immuno)fluorescence stainingLaboratory (Neugasse 25)
1.00-4.00 pmD, PFluorescence and laserscanningLaboratory (Neugasse 25)
Thu, Sept 15 Routine Measurements and AnalysisInes Hilke, Sonja Rosenlöcher, Birgit Fröhlich (MPI-BGC)
9.00-10.30 amLHow to determine carbon, nitrogen, and other parameters in solid and liquid samples?
  • overview of analytical methods (e.g. elemental analysis, sum parameter analysis, flow injection analysis, and ion chromatography)
  • no limits? - instrumentation, pro and cons of application
B0.002 (MPI-BGC)
11.00-12.00 amDDemonstration of analytical methods and laboratory instrumentsA1.019 (MPI-BGC)
13.00-16.00 pmPAnalytics of solid and liquid sampleA1.019 (MPI-BGC)
Fri, Sept 16 Volatile organic compoundsGeorg Pohnert (IAAC, FSU)
9:00-11.00 amL"Headspace SPME, GC/MS"

Basics, enrichment of compounds from the gaseous phase → analysis

Seminar room #127 (Lessingstr. 8)
11.00 am - 1.00 pmPLab class "DMS from water samples" as an example + discussion & transfer of results (group 1)Lab (Lessingstr. 8)
1.00-3.00 pmPLab class "DMS from water samples" as an example + discussion & transfer of results (group 2)Lab (Lessingstr. 8)
Mon, Sept 19 LA-ICP-MSNeele van Laaten (FSU Jena, IGW)
9.00-11.00 amLTheory of LA-ICP-MSSeminar room (IGW)
11.00-12.00 amDPractical applications of LA-ICP-MSLab (IGW)
Wed, Sept 21 Principles of Good Laboratory PraxisDr. Michael Raessler (MPI-BGC)
9.00-11.00 amS
  • Lab organisation
  • Documentation starting from arrival of samples
  • Helpful advice for 'customers' of analytical facilities
B0.002 (MPI-BGC)
Wed, Sept 28 Extraction methods and associated techniquesMolecular Biogeochemistry group (MPI-BGC)
9.00-12.00 am


1.00-4.00 pm
L, D
  • Extraction
  • Column and gas chromatography
    • liquid/liquid extraction
    • solid phase extraction
    • distribution ratio
    • selectivity
    • reading chromatograms
  • Recovery rate
  • Calibration
  • Quantification of alkanes
B0.004 (MPI-BGC)
Sept 29-30 Raman spectroscopyTorsten Frosch (FSU Jena / IPHT)
Thu, Sept 29
10-12 am
LRaman spectroscopyB0.004 (MPI-BGC)
Thu, Sept 29
1-4 pm
D, PLab class: Raman gas spectroscopyLab (IPHT)
Fri, Sept 30
1-4 pm
D, PLab class: Raman microspectroscopy of mineralsLab (IPHT)
Tue, Oct 4 GasLabArmin Jordan & Daniel Rzesanke (MPI-BGC)
9.00-11.30 amL
  • Principles of trace gas measuring techniques
    • Spectroscopy: laser-optical gas analysers, FTIR, NDIR
    • Chromatography: GC
  • Requirements for high-precision trace gas measurements (Standardisation, quality control)
  • Sampling —> Laboratory analyses
  • Critical consideration of interferences
B0.002 (MPI-BGC)
12.30-13.30 pmDTour through the Trace Gas Analysis and Gas Standard Preparation Flask and Calibration LaboratoryCAL-FCL (Kahlaische Str. 4)
Tue, Oct 11 14C AnalyticsAxel Steinhof (MPI-BGC)
9:15-10:30 amLIntroduction to Accelerator Mass Spectrometry (AMS)B0.002 (MPI-BGC)
10:30-11:00 am Coffee breakfoyer, ground floor (MPI-BGC)
11:00-11:30 amDLab demonstrationC01.005 (MPI-BGC)

3.  Material

Presentation of Katrin Krause: Analytical techniques in microbiology
Presentation of Ines Hilke: Routine measurements and analytics
Presentation of Georg Pohnert: Volatile organic compounds
Presentation of Neele van Laaten: LA-ICP-MS
Presentation of Dr. Rässler: Good laboratory praxis
Handout of Vanessa-Nina Roth: Extraction methods and associated techniques
Presentation of Torsten Frosch: Raman spectroscopy - will be uploaded soon
Presentation of Armin Jordan & Daniel Rzesanke: Introduction
Presentation of Armin Jordan & Daniel Rzesanke: Trace gas measurements
Presentation of Axel Steinhof: 14C Analytics


4.  Feedback

The survey results are presented here. Statistics and statements should not be taken as an exhaustive or exclusive list.

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